FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005005391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53443923
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC276300.09.712529
GATCGGA292750.09.4855511
ATCGGAA313650.08.970522
AGAGCGT313400.08.7704838
TCGGAAG336200.08.4329933
CGCCGTA103700.07.825346555-59
CGGAAGA366250.07.80027684
AAGAGCG387600.07.46474747
GCCGTAT125600.06.57606255-59
TCGCCGT122550.06.562676455-59
AGCGTCG225550.05.6763529
CGTATCA151350.05.59106160-64
TGGTCGC157350.05.414737750-54
GTCGCCG146850.05.397915455-59
GCGTCGG62700.05.1909256145
GTGGTCG168700.05.050438450-54
Produced by FastQC (version 0.11.5)