FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50008741
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAAATCATCTCGTAT565540.11308822991564615TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA244500.040.4059871
ATCGGAA259850.038.4445232
TCGGAAG280500.035.7101753
CGGAAGA294550.033.804844
TATGCCG120350.017.47218545-49
AGAGCAC585700.017.1220118
GAGCACA637900.015.8458479
GAAGAGC656100.015.6382116
CTCGTAT132900.015.3649940-44
TCTCGTA132900.015.23366540-44
CGTATGC136450.015.20881745-49
ATGCCGT151650.013.62708845-49
TCGTATG148700.013.4881145-49
ATCTCGT161550.012.49604740-44
GCCGTCT170550.012.1510150-54
TGCCGTC168600.012.0164950-54
AAGAGCA905700.011.6245237
CGTCTTC198650.010.96474450-54
CATCTCG192450.010.64837340-44
CCGTCTT203650.010.3041550-54
Produced by FastQC (version 0.11.5)