FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15995816
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC80250.030.322389
AGAGCGT86000.028.4629928
GATCGGA86700.028.4019361
ATCGGAA90350.027.0230962
TCGGAAG98750.024.8617483
CGGAAGA105300.023.3144664
AAGAGCG106950.022.8201337
CGCCGTA36050.013.90366755-59
TCGCCGT40100.012.57168255-59
GAAGAGC216600.011.9030036
GCCGTAT43550.011.542555-59
GTCGCCG45550.011.06749755-59
CGTATCA47800.010.69757360-64
CCGTATC46600.010.5689460-64
TGGTCGC51150.010.0247350-54
GGTCGCC51550.09.5826255-59
GTGGTCG55850.09.36265750-54
GCGTCGT59700.08.29715910-14
CGTCGTG60600.08.22173410-14
TCTCGGT70150.07.723427345-49
Produced by FastQC (version 0.11.5)