FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52021851
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC309150.019.6439869
CGCCGTA76950.018.71430455-59
GATCGGA354550.018.5153331
AGAGCGT341000.017.9363148
TCGCCGT88700.016.23523955-59
ATCGGAA394150.015.6131882
AAGAGCG396000.015.5910997
TCGGAAG399550.015.3646443
CGGAAGA413550.014.8945594
GTCGCCG101200.014.64380755-59
GCCGTAT103750.013.96368355-59
TGGTCGC113800.012.93252150-54
GTGGTCG124750.012.08679750-54
CGTATCA127650.011.45068460-64
GGTCGCC119700.010.17233455-59
CCGTATC120400.010.16081760-64
TCTCGGT167700.09.38748745-49
GCGTCGG33950.08.720914145
GAAGAGC761250.08.5467956
CTCGGTG184300.08.54195145-49
Produced by FastQC (version 0.11.5)