FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005005514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67292869
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA349300.017.4850581
ATCGGAA373600.016.466332
TCGGAAG383900.015.9188953
CGGAAGA405850.015.1115234
TATGCCG115250.012.04052245-49
CTCGTAT134500.010.23202840-44
CGTATGC132350.010.19999645-49
TCTCGTA136150.010.15063140-44
ATGCCGT158000.08.97544745-49
AGAGCAC760750.08.4522788
GCCGTCT167750.08.21097750-54
TCGTATG154050.08.13254245-49
GAGCACA825500.07.9737189
TGCCGTC162950.07.8122150-54
ATCTCGT175500.07.74248340-44
GAAGAGC868450.07.51259766
CGTCTTC207500.07.224972750-54
CCGTCTT211350.06.80523650-54
AGATCGG342900.06.724143145
TAGATCG138350.06.0793366145
Produced by FastQC (version 0.11.5)