FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328443
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3855.610018E-616.7322337
ACACGTC4151.9843447E-413.7979328
AGAGCAC6900.00130340159.3375063
CTCCGAT10900.0099802696.5974691
CAGCTGT12350.0048658146.3762193
CTTCTAC13000.0079091496.0848971
GAGAAAC13050.0086376096.03329046
ATTCAAA22857.9553627E-45.03544241
Produced by FastQC (version 0.11.5)