FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624210
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCG3100.005756972414.0630911
AATCCAC6000.00417116149.59009555
CTTCTGA7702.9409718E-49.4362731
CTTCAGA6350.0061205129.1539281
CCTTCTC7350.0020209638.8970571
CCGATTT8450.00778930777.66072753
CTTTGAT10850.0010615397.36638071
CTTCCAT11100.00135206597.20047141
TTTATTA13502.1994175E-46.9261813
CTTTTAT11600.0021543386.89010571
CTCGTCT2450.0036162556.56062115-119
ATTTTGA16300.00214850275.7364076
Produced by FastQC (version 0.11.5)