FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085456
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGG16004.9502523E-77.69768241
GTCTATT9800.00331076587.3927321
GTCCCAT12707.6700613E-46.84555341
CCCAAAT13300.00129523336.53673121
CTTGATT22201.9519666E-66.20057061
CTCAAAT14850.0044602015.85444641
CACAAAT17507.303269E-45.7959021
CTAAAAA20351.3819078E-45.6962181
CTTCAAA33251.3380486E-85.6651681
CAACGAT19906.26418E-45.4609631
CCAATAC17350.0042643025.41555073
CTTTGAC26858.3485265E-65.3965571
CTCCGAT17700.0052451575.32109641
Produced by FastQC (version 0.11.5)