FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555873
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAA6907.811379E-611.5598671
CTTCAAT9251.7205839E-610.1908611
CTTTAAA8406.847849E-59.49560451
CCCGGAT6400.00663594859.0639861
CCTTGTT6500.0075363858.92454051
ATTCAAA9050.00149771938.0123631
CTCCTTT8750.0098890217.4583661
CTTTCAT11100.00138083727.1858631
CTTTGAT10200.00483501847.10900931
CTTCAAA11400.00183109016.99676131
CTTTCCG10550.00670759056.8731651
TTCAATA12850.00673224646.17874962
Produced by FastQC (version 0.11.5)