FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009975
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA5007.559483E-411.7748151
CAATCGA5400.001439505110.9026061
CACCCTT6900.00101202559.5990341
CTCGAAG7250.00159022139.13563251
CATCGGA7800.00308869068.4914531
CCCTTCA8558.9260936E-48.444093
ATATGCG8100.00515914438.0218868
CCCAAAA11151.4317484E-47.9202791
CAACAAT12104.2828844E-57.90664241
CTTCAAA19451.5033947E-77.18899351
GATATGC11850.00288792866.70182857
CACAAAA15307.652166E-46.25296551
CTTCTCC19405.507266E-56.06949231
CTATTAT15150.0046735785.8291161
CCATTTC15500.00743940475.58944274
CTTCAAT17150.00299928835.57844731
CTTCCTT17400.00355959685.4982971
TTCCGGA17800.0058282495.27281248
CATCCGC18350.00665157475.2136441
CAAAAGA24102.1785063E-45.19117831
Produced by FastQC (version 0.11.5)