FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876764
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3300.031.3847777
ACACGTC3650.030.2669648
CACGTCT3850.028.6946549
AGCACAC6800.017.2606455
GCACACG6054.1836756E-1117.1179926
AGAGCAC7701.0186341E-1014.3497823
GAGCACA7851.382432E-1014.07477954
GTCTGAC4200.00435901211.5353831
CCCAGGG4250.004761429511.39967251
GAAGAGC11706.53381E-910.0564881
AAGAGCA13901.1108568E-78.4483922
GTTGAAG9253.327228E-48.2104676
AGTCACC4051.2758574E-76.81979720-24
GAATATA12050.0054761896.30371954
AAGGCAA12100.00572744946.27659755
CAGTCAC5051.0899639E-65.742809320-24
CGTCTGA5858.596362E-85.6652910-14
TCGTATG5457.537601E-75.574651240-44
CCAGTCA6008.4536805E-75.293867620-24
GCACTTA5003.2431082E-55.24867230-34
Produced by FastQC (version 0.11.5)