FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15706669
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA44600.045.51722145
GTAGATA279300.033.408745145
CGTCGTT51400.030.750055145
TCGTGTT143000.029.051668145
TAGATCA148200.026.173279145
GCCGTAA38350.024.577093145
GCGTCGG174150.023.314014145
TCGGAAA70550.022.814367145
TCTCGGG137650.020.699928145
AGAGCGG320450.019.910168145
AGTGTAA138700.019.654587145
GGGAAAA405200.019.234972145
AAGAGTT266200.019.228666145
CGGTGGG169650.017.607426145
GAGTGTT153500.017.570621145
AGTGTAT169000.016.602627145
CGCCGTT35550.015.499814145
TGTAGAA254100.014.123849145
TCGCCGG94750.012.855311145
TCGGAAC60550.012.692414145
Produced by FastQC (version 0.11.5)