FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17136174
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA42950.052.34307145
CGTCGTT51700.040.959347145
GTAGATA272700.038.826515145
TAGATCA153200.032.18925145
TCGTGTT130950.030.73606145
GCCGTAA37250.028.22945145
TCGGAAA80700.025.970783145
AAGAGTT276700.022.828096145
GGGAAAA430550.019.97661145
GCGTCGG157500.019.707153145
AGTGTAT166200.019.635511145
AGTGTAA148650.019.172924145
AGAGCGG282950.017.607906145
TCTCGGG130300.016.919592145
CGTGTAT123850.016.21976145
CGCCGTT35850.015.373938145
GAGTGTT152450.014.746704145
TGTAGAA255550.014.558016145
CGGTGGG166400.014.164157145
CGTGTAA60000.013.174555145
Produced by FastQC (version 0.11.5)