FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463317
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGCG4802.4049987E-615.0934631
CTCCATT4150.002828134212.220251
CTCATTT4907.2640873E-411.8283451
CTCATAT4650.006611586510.9062431
CCTCTTT8300.0062118947.8558751
TGCATTT8350.00684817287.77088982
AGATTTT10659.4685116E-47.4449884
CTTGAAA10000.00401814937.2448621
Produced by FastQC (version 0.11.5)