FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514919
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTCT3859.5901756E-515.0264831
CTCCCAA4550.00570354211.1253771
CATAGAT4800.00848687210.5459291
CTTCAAT9301.9080126E-610.1085411
CTTATAT6607.8923255E-49.861131
GTTTGTT7954.2448795E-49.096221
CTTAAAA6450.00723193878.96929551
ATTTACC6500.007834948.8821392
CTTCAGA6650.0092876358.6995431
CTTCCAT9250.00190754187.8178321
TCTCCAT8650.0093105917.50874423
CTTTGAA10250.005204687.05511671
CTTTCCG10700.00789140356.7584061
TTTGTTT17155.897187E-45.89121442
Produced by FastQC (version 0.11.5)