FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647933
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2200.038.673617
CACGTCT2800.032.918619
ACACGTC3505.456968E-1226.3348878
AGCACAC3951.8189894E-1225.1277485
GAGCACA4507.2759576E-1222.056584
AGAGCAC4651.2732926E-1121.3417823
GCACACG4351.1277734E-1021.1873536
GAGCTAT3200.00836637513.2909763
GTATGCT4504.17084E-412.6359441
AAGAGCA7651.9782783E-712.0505032
GAAGAGC8959.511496E-911.9123781
AGTCACG2551.8077117E-89.4528220-24
CGGATCG7300.00237784058.7392723
CGAAAAT8206.87388E-48.6679491
AAGGAAC8250.00713818477.73591662
CAGTCAC3155.4444536E-77.65228220-24
CTCGTAT3401.3800918E-56.672579335-39
ACGTCTG3102.0280882E-46.40312810-14
GTCACGT3358.050087E-56.348909420-24
CACGTCA3358.062097E-56.34822325-29
Produced by FastQC (version 0.11.5)