FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883991
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATGT4050.002226725712.6180351
CTCTGCT8002.4285146E-711.8632061
CTTGGAT4450.00450719911.48382951
CGGTCCA4700.00732526610.7585949
CTCAAGA6153.7799703E-410.6835631
CCCAACG4800.0079175910.6464661
CTCAAAG7702.448342E-510.4291931
CTCCCAA4900.00922543310.4291931
ACCAAGG5750.002832532210.0502637
CTAAAAT6607.2447467E-49.9551381
TGGGATA6000.0040225469.6331382
CCTCAAA8406.370415E-59.5600931
CTTCGAA7803.1954524E-49.3595321
CTTTTAA11753.486275E-79.3197031
CCCATTT9502.4119832E-59.22160151
CTCCAAT6350.0058907859.1973981
GTTTCAA10608.972775E-68.9533631
CTCCGAA8050.00441368678.1619761
CTTAAAA9400.00203894157.76641941
CTTTTAT13251.7481143E-57.71366641
Produced by FastQC (version 0.11.5)