FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558652
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT2851.7310545E-417.6694761
CTCTGAT4703.524557E-513.7757441
CAGGGAG4150.002982735612.1344581
CTTACAA4500.0054612511.1906671
CCTCAAT5800.00314540239.922761
ATTCCAA8400.0080661797.63057858
AGCTTTT12356.754155E-46.9200394
AGAAATT11700.00290971786.6957794
Produced by FastQC (version 0.11.5)