FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3296663
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAT8255.9688103E-48.79412651
CTCTAAC9703.2608717E-48.2274951
CCCCCTC11500.00200388256.93971251
CTCACGA13902.8408424E-46.7853961
CTCTAAT15351.3228352E-46.6170791
CCAAGGG17258.7848195E-56.308831
CTTTGAT60050.06.28256461
CTTCAAT56800.06.13111641
CCCATTT34601.382432E-106.08090971
CTTCGAG16904.5920713E-46.0101871
CCCACAA24151.2526107E-66.0084091
CCCATCT24201.2997043E-65.99599551
CTCATGT15750.00129064995.9883821
CCTTAAT20802.9251505E-55.9296931
CCCACTT18402.1281463E-45.91452841
CTCAAAT29453.4966433E-85.91251951
CTTTCAC39752.1827873E-115.84062771
CCCAGTT22451.5060052E-55.817051
CTCAGAA20101.1343046E-45.7752471
CCCCAGT15100.0052873365.7656851