FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469134
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATT14256.89397E-109.7241581
CTCAGAT11502.5434494E-58.2443961
CCCAAGC10600.00665980346.8803011
CAACAAT32001.8189894E-126.83729931
GTTTCGC14808.693194E-56.8329566
CTGGTTA10650.0076395016.7833694
GTTTGAT25952.0918378E-96.74508141
GGTTAAT11000.0095238586.63010841
CTCAAAC15501.4022486E-46.58733371
CTCCAGT15651.5895203E-46.5241961
CTTTAAT40250.06.5230381
CCCAGGT12350.0040106396.49589541
ATCACGA16553.2790346E-46.1694061
CTCAGAA15550.0010393476.09714171
GAGTTCG14300.00302735886.0616035
CTCATGA14500.00317585176.03568461
CTTACAT15950.00140988275.94423531
CTACAAT22201.1552933E-55.91333961
CTTCGAT23554.7661943E-65.8840451
CTTCATT41007.2759576E-125.87007141
Produced by FastQC (version 0.11.5)