FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393587
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACAT9500.00254210397.5945851
CTCAGAA9550.0026763197.5548231
CCCTTCT9950.0039966737.25111151
CTCCGAT11050.00139209777.18220951
CTCTGAT10550.0070575666.8387261
GGAAGAC10700.0080893046.7428561
GAAATAG18503.928403E-56.2125595
CTTTCCT21000.00135800035.15346861
CATAAAG19800.00364931185.10141321
Produced by FastQC (version 0.11.5)