FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871972
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC3300.009153509513.1119882
CTTCAAA15050.011.0629261
CTCATAT8355.7648576E-610.4033561
CCCAGTT9301.8884548E-610.1190351
CTCGCTT6506.804828E-410.0232331
CTTAAAG5800.00298999349.984831
CTCCAAT8507.952286E-59.368121
CAACTGA7200.00173436839.0487521
TCCGTAT8000.0046712628.1107173
CTTTCGA8100.0050353118.0433351
AACGATT9550.0026857697.5514072
CTTCGTT10250.00516103957.06244041
TCCAATA10600.00741361176.803392
CTTCAAC14909.203215E-56.8017461
CAAACAA10850.0089507676.6718911
CTTCATT17158.330378E-56.33148861
CCATCAT22051.7543771E-66.2376881
ATCAGAA20552.503352E-55.98846861
CTTTCAT15050.0052117655.7719611
CAGATTT15200.00608610835.6917313
Produced by FastQC (version 0.11.5)