FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543159
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCGG2250.0046377819.880049145
CTTAGCT2000.00770676218.2075941
CTCAAAT5601.1236927E-513.00542451
CATCAAT10103.2014214E-911.5374861
CTTCAAT9201.2688906E-711.0828831
CTTTCCT6506.4273615E-410.0842061
CCTTGAT7150.00153821939.16745951
CTTTTAA9700.00283643257.50828551
CCATTTT10800.008846916.67899234
TTCAATA12300.0042975696.45092872
AGCTCTG4305.292003E-55.703548465-69
Produced by FastQC (version 0.11.5)