FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63659358
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATTA537450.020.8716891
TGTTCCG526450.020.76441
AGTTGTG415250.020.713757
TCACATC553100.020.6341975
TGCCGTC497850.020.5934832
CTCGTCA724100.020.4872071
GTTGTGG494300.020.4289138
TTGCCGT520150.020.4149571
ACATCTC543550.020.4148087
AGGCCAC526050.020.3980541
GGCGTTC383650.020.3746853
AGCTTGA291800.020.2431961
GGTATTG438150.020.207281
TAGACCA426550.020.1871431
TGAGCAT546500.020.1723671
TCGTCAC548750.020.1595212
CCTACCA475500.020.0880641
CATCTCG553050.020.0601888
GCTGCAG475850.020.0570431
CACATCT551950.020.035776
Produced by FastQC (version 0.11.5)