FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324288
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGG2000.00806412818.0673528
TTGGACC3250.00815474813.3440622
CTTTAGA6606.3164225E-510.9897791
CGTTAAT5900.00339092389.8349211
CTATAAT12951.4068048E-57.84135531
CTTTGAT20901.4915713E-97.63500451
CTCGTTT9500.0024129577.6350041
CTCTTAT9500.0024129577.6350041
AGACATA8550.0083044167.60730554
CCTCCGT9550.00254043777.5950311
AAACTAA9600.00277078017.52806335
TCAACAC9650.0029110437.49018962
CTTCAAC14659.0051635E-67.42653941
CTTTAAC9800.00327166757.40127941
CTTAAAT10300.0053128057.04199461
CTCATCT11650.00230097726.8485661
CTTGAAT12808.2700106E-46.7999261
CGAAAGA16253.8487524E-56.6953121
CTCATTT12150.00357738816.56673241
CTTCTGA17952.1910568E-56.46529631
Produced by FastQC (version 0.11.5)