FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433198
Sequences flagged as poor quality0
Sequence length100-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACACAGCAACATCTCGTATGCCGT6530.1507393847617025TruSeq Adapter, Index 5 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2250.063.3752759
CACACGT2550.055.9193577
ACACGTC3650.039.066958
AGCACAC3750.038.0251665
AGAGCAC4050.035.2084853
GCACACG4100.034.7791186
GAGCACA4100.034.7791184
AAGAGCA5900.024.1713262
GAAGAGC7900.019.0050681
CTTTTAG1900.006479291218.7624175
ATGTACA2950.004693920714.5011228
AGTCACA2150.013.93103920-24
TTCTACG3300.00985952312.9631244
GCACTGA3300.00985952312.9631244
CAGTCAC2750.012.44747320-24
GTCACAC2550.012.305099520-24
TCACACA2600.012.06651325-29
CTCGTAT2509.458745E-1110.83742135-39
ACGTCTG3001.8189894E-1210.45692110-14
ACACAGC2807.094059E-1110.18601725-29
Produced by FastQC (version 0.11.5)