FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290501
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAT4108.557097E-616.0049551
CTCAAGT5104.2377887E-614.2963661
CTCTGAT10353.6379788E-1011.9757971
CTGACGT5008.1756664E-411.6658351
CTTACGA7100.00143111549.2422991
CCCAAAT8200.00527463538.0024781
CTCATAT11201.6808198E-47.81194351
CTCAAAC9450.00217677197.71549941
CTCTGAA12356.208694E-57.67489151
CTAAAAT13402.0681697E-57.61761671
CTTGTAT13051.2291993E-47.26321171
CCTCTAT10150.0043786547.1833961
CTTCAAT21009.140604E-86.9439491
CTCACCG11600.00208136976.9140181
CTTTGTT20103.142868E-76.89212941
GTTCCAT18103.1350555E-66.84803871
CTCTGTT12858.146879E-46.80885361
CTTTAAT18353.8858434E-66.7547411
CTTCTAC13009.296059E-46.73028951
CTCTTTG17451.3386718E-56.68529221
Produced by FastQC (version 0.11.5)