FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144690
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT9602.0814696E-710.6957781
CCCACAT7351.6788465E-49.9785681
CTTAAGA7850.00337536338.4086911
CTCTGAT9803.2353887E-48.2323191
CCCTCAT8350.0058860717.90517761
CATCGAT10406.1249424E-47.75737761
CTTCAAG15301.7264938E-67.66980171
CTCTGAA9950.00340395987.37110331
CGATGAT16957.97511E-66.9231841
CTCCCGG10800.00754969666.79097031
CCCTGCT14052.831415E-46.78613661
CTTTGTT16855.4541455E-56.5290041
CTTCTGA14704.913587E-46.4860691
CAATGGA13600.00144928096.4713951
CTTCCAC15358.2967966E-46.21141531
CCTCAAA13000.00644673836.20590161
CATTGAT20351.7646042E-56.12688971
ATTCTGG15400.00109950276.0679158
CTTTTCT28751.06618245E-75.86739831
CAATTAT15150.00485526355.80930571
Produced by FastQC (version 0.11.5)