FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771999
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAG6009.7439624E-814.5765111
CTACGAT4500.00495933211.3372881
CTTTAAT11001.0059011E-911.2636691
ACCAATC6709.277861E-49.6899972
CTTACTT6050.0043621999.538733
CTCAAAA12306.816845E-89.4806581
TCCGATT7800.0036979878.3234592
CTTCGGA9150.00158902497.96530681
CTTTAAA14204.4709057E-57.1856041
CAAGAAG11900.00273109626.73704341
CTTATTT13009.3238044E-46.72762061
CTTCAAT14153.329641E-46.69592431
CTTCAAA15952.0446818E-46.39721541
CTGAAAT12850.0060943856.2389741
TTCTTCG13000.0076484716.10386942
CTTGAAA14650.0035801645.9699031
CAAAAAT18400.00127662965.54541251
TCAATAA15650.0083420755.5312443
GTTTCTT18750.00161990225.4418981
AAAGATA24858.2697756E-55.22518974
Produced by FastQC (version 0.11.5)