FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777375
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAAT4205.874299E-717.2799841
GGCACGT3250.00793378813.3986351
CTCAAAT5651.2782715E-512.8452991
CTTAAAG5008.491466E-411.612151
CATAAGG4750.007651318310.6954011
CCCTGAT7301.7407608E-49.9419091
CTTTGTA7702.9772348E-49.4254461
CTTTTGG9302.010142E-59.3646371
CTCCCAT6650.0090264468.730941
CTTTTCT16951.8681021E-98.5635331
CTTCAAT11653.170425E-58.0986021
CCTTCCT9250.00184369137.84604741
CTTCTAT9250.00184369137.84604741
CTCCTTT13001.4660387E-57.81587031
TTCTATT12306.501073E-57.6454392
CAACAAA11402.1725398E-47.6395721
CTTTCAG13352.088044E-57.61095951
CTTCAAA15302.0176994E-67.589641
CTTTAAA10658.8222075E-47.4961061
CTTTATT15351.7179109E-57.09211161
Produced by FastQC (version 0.11.5)