FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816865
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAGA3753.5477133E-617.5551131
CTTAAGA4755.167554E-412.3193771
TAAACCT4500.005304568411.2355289
CTCCATT7852.9636754E-510.2498011
CCTCATT5850.002945114410.0029131
CTTTAAA12951.3915269E-89.6022181
CTTCGAA6250.0050865799.3627261
CCTCCCG6250.0050865799.3627261
CTTTCGA6350.00579659139.2152821
CTCAAAG8759.739291E-59.1955361
CTTCCAT13603.090281E-89.1432881
CCTTTAT10457.306244E-69.099541
CACCAAC7300.00178859519.0180381
CCTCGAT8205.167675E-48.920281
CTCATAG7450.00215189118.8364671
TGGTTTC8407.4417575E-48.5996714
CTTTATT15603.1468517E-88.4399581
CTTGTAA8300.00570933157.93152671
TTAAATG8200.00572608747.92896272
CTTGTTT10950.00109111417.34803061
Produced by FastQC (version 0.11.5)