FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316002
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGACT6250.0056580289.2432131
TCCGATC8951.4679004E-48.8564023
CTTCCGA12701.1503444E-57.9604441
CACGGAT10205.876905E-47.78763341
CTTCGAA10800.00108149817.3549871
CTCAAAA16657.708943E-66.93934871
CTTGTAT12700.0059454556.2546351
CTCCGGT12800.0064501646.205771
CTTCAAC16353.180752E-46.18334151
TTCCGAT18804.7428806E-56.13267332
CTTCAAT22803.246736E-66.01771641
CTTCCGC18201.9504956E-45.9515881
CTTGAAA25303.100493E-65.7085061
CAGCTGG15350.00667030045.64528471
CTTCTCT15600.0079718885.55481531
CTTTTGG15900.0098300065.45000741
CTTTTGA22951.3926212E-45.34908151
CTTTCAG24858.158677E-55.23069141
CAAAGAA33154.5415436E-75.22806171
CTTTGAT21350.00167102065.0734851
Produced by FastQC (version 0.11.5)