FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095830
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGCT7301.8334993E-49.8916961
AGTCGAC1300.0070526078.999602115-119
CCTACAT8558.9123286E-48.4455421
CCCATAT10751.1678845E-48.0605821
CCCGAAT8150.00544480057.9740421
CCCAAAT10105.2886043E-47.8643881
CTTCCGA11803.4310058E-47.3433271
CTTCAAT20658.015013E-86.9936451
CTCCAAT10450.00638111456.90998841
CTCAAAA15802.7675345E-56.85532141
CCCCCCC2650.0096169425.971654410-14
CCTTGAT15750.00136321115.96013931
CCATGAT15600.0079724685.5545681
CTTCAAA22501.03359554E-45.455821
CTTTAAA19050.0022263125.3067261
CTTTGTT20800.00118138125.2074071
CTTTATT29709.346808E-65.1057141
GTTTTCT19850.00372390385.0928531
TTTAAAG19900.00402137645.0615092