FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851677
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAAGT4550.00652103910.9293257
CTCGTGA6300.00669804089.0557541
CTCGAAT8206.6647265E-48.6968361
CTTGGGA14006.910868E-78.1501791
CTCGAAA17052.5785994E-87.94702151
CTCCCAC10005.438988E-47.8445471
CTCGCAG8350.007572447.68654541
CCTCGGT8600.0098549057.46311
CTCCCCG11450.0022931426.85113241
CTGCGCT11800.0031468336.64792061
CTTCCGT25853.1177478E-96.62103461
CTTGTAA16455.7754558E-56.50280241
CTCAATT12250.0046547016.40371131
CTACCGT13700.0021609586.2464871
CTTCAGT14958.4725564E-46.2012231
CCCCAAT12750.0070563686.15258551
AAGGTTG15150.00104010926.0965535
GTGTAAT12900.00796522856.08104371
CTTTGGT22401.9128409E-55.73059371
CTGGCAT15050.0061624695.6861711