FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584163
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAC6150.0046247729.4724951
CTTACGC6200.0049439989.3961041
CTCAACT8004.2281114E-49.1024761
CTCGCTT8306.105844E-48.773471
CTCAGGA9452.3618559E-48.4763791
CTTAAAG12206.005128E-68.3563711
CTCCAGG7850.00360224688.3487671
CTTTAAT22359.094947E-128.1453921
CCCACAT10751.05983E-48.1287221
CTTGCAT13653.1256059E-68.0021761
CTCAAAG15753.5465928E-77.85991571
CATGACT8400.00662413337.80212161
CCTCATT14205.479975E-67.69223261
CTTAAAA19151.7178536E-87.60520171
CTTCTAA8700.0090661727.53308341
CCCCAAT10708.962977E-47.4861481
CCCAAAT12901.0828608E-47.338431
CTCCAAT14901.0818156E-57.3308531
ACAATCG10050.0044036897.1794263
CTCAAAA20506.024857E-87.10437151
Produced by FastQC (version 0.11.5)