FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651405
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGA1850.00496758719.6470551
TAAACTA2900.003839645814.9477744
CGTAGGT3250.00784708913.420451
CTCCCGA3350.00958623113.019841
GTCAACG5700.002498528510.2026811
TCGATTT6200.0052689829.32226853
ACAATTT8105.1690626E-48.9194553
CTTCCAT11051.5980097E-58.5522471
CAAAGAT9100.00154358367.98836331
TTCCAAT8700.009685447.4761842
TCCAGAT12204.9721944E-47.1085032
CTTTCAT10600.00685691976.8579341
ATTTCTC10550.00696316646.847066
GAATATA10700.0079695086.75211054
TTTCCAT16350.00212111955.74269258
GATTTTC16800.00292345355.58972845
Produced by FastQC (version 0.11.5)