FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449279
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACGACGAATGATCTCGTATGCCGT8470.18852428001308763TruSeq Adapter, Index 6 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2800.061.993857
ACACGTC2800.059.5140958
CACGTCT3100.053.7546659
AGAGCAC4650.037.3337863
AGCACAC4900.035.425065
GAGCACA4750.035.08594
GCACACG5100.034.035846
AAGAGCA6700.026.950192
GAAGAGC6950.023.1453041
ACTCGAA1800.00552565119.2869745
CACACGC2600.002413710616.0248093
TCGAACA4202.8348036E-413.2253547
AGTCACG2550.013.1027220-24
CAGTCAC3100.011.22712820-24
CTCGTAT3150.010.60081335-39
CCAGTCA3200.010.44122920-24
TCTCGTA3055.456968E-1210.036015535-39
GTCACGA3750.010.02358120-24
ACGTCTG3750.09.99836710-14
ACTCCAG4200.09.91901615-19
Produced by FastQC (version 0.11.5)