FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995850
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA6657.6660427E-49.8942971
CCTCCGT6150.00447410169.5099651
CTCAGGA6350.0058236169.210441
CTTGAAT11402.2064774E-58.3368611
CAATAAG10951.2528547E-48.011821
CCCATCT8250.00543549477.97540241
CACAGAT10255.423643E-47.84572121
ATTAGAT8400.0063895567.8329851
ACAAAGC10507.981224E-47.56737473
TGTTATG9850.00357789647.33340367
CTTCAAA21051.1787051E-87.29342031
CTTCAAT16254.463507E-67.19831231
CTTGATT14555.922611E-57.0344331
CATCAAT17852.4132041E-66.96265271
CTTTGTT16002.0481259E-46.3969381
CAAAAGG14200.00244179276.1781291
TAAAGGT14450.00339603215.99867343
CTTTCAT20902.7897877E-55.94657231
CTTTAAA18802.562832E-45.83307361
CTTTCAA17808.0655474E-45.75005671
Produced by FastQC (version 0.11.5)