FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538499
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAA8659.911876E-59.1826379
ATAGCGA11853.607728E-47.3122968
CTACAGG12004.045535E-47.23971461
CCCAGCT19306.5000066E-56.0018371
CTTAAAC16300.00199529955.7740061
CTTTTAT59450.05.72357651
GATTCGG17950.00105272565.6318935
CTTCCGT29802.869656E-75.58769941
CGTTAAA15600.0079843285.5545325
CTCAAAT27402.0543303E-65.5486871
CTTGGAA32102.2210406E-75.41287141
CTTGTAT25451.9889232E-55.40489531
ATCGAAT21803.7320209E-45.31355241
CTCCAGC31609.2533264E-75.2694131
CTTGTAG19250.00246119785.26524731
CTATTGT19350.00262627845.23803661
CTTTGAC29106.1815244E-65.22453641
CTCCCAT18450.0085970745.10115431
GTTAAAT21400.00166874655.0745671
CTCCGAA20050.0040920125.05516241
Produced by FastQC (version 0.11.5)