FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3423662
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAAT31750.08.6887981
CTTAGAT9350.00204786947.76440571
CTCAAGT11150.00143571737.16205451
CCCAGTT28909.094947E-127.0336381
CTTCCTT70550.06.997321
CTCAAAA46800.06.98049931
CTCAAGA20003.1173477E-76.8967331
CTTAAGA14808.144086E-56.86730151
CTTGTAT41700.06.78966571
CTCAGAT14253.819093E-46.62290141
CTCCGAT27501.1641532E-96.5997451
CCCCAAT21107.8815174E-76.53718761
CCCAGGA15551.55302E-46.5360821
CTCACAA17801.9161282E-56.52559041
CTCAAAG31508.54925E-116.4530831
CCCAGAT27156.1154424E-96.41743141
CTTACTT24955.0851668E-86.40135531
CTCTAAT13650.00169797286.38217071
CTTGAGA17158.0383965E-56.34960841
CTTCTGT51550.06.33729171
Produced by FastQC (version 0.11.5)