FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272355
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAC5950.0038392529.6888778
CTCGAAT8256.143524E-48.7684351
CCCGAGG9850.0035314397.344121
CTCAGAA12851.12081965E-47.3184011
CTTCGAA15001.30581175E-57.23395821
CTCGCTT12054.281468E-47.20394131
CTAAAAG11900.00296055136.68685151
CCCCGCT14555.294596E-46.4470962
CCCGGAG22302.163497E-66.1634621
TTAAACT14850.00473365845.82324173
CTTAAAT19053.5476533E-45.696031
CTCAAAT16650.00259320835.6481351
TTTTGGG23102.6513857E-55.6151457
CTTGAAC19354.3826635E-45.6077191
GTTTAAA18700.00172652935.41579771
CACGAAA17500.00467181865.37379741
CTACAAC18850.00190929025.3727011
CTCAAAG20459.21378E-45.30608221
ATTTGGT19700.00331821155.1408841
ATTTACT22556.427006E-45.1129776
Produced by FastQC (version 0.11.5)