FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535938
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAAG3705.943412E-515.8928581
CAAAGTC4750.006956983410.8322381
CCCGCCT4950.00944708510.3945711
CAACAAG7350.0018182829.0005471
CTTAAAA6750.0091832588.711641
GGGGGGG1600.0046478258.1164910-14
CTTTTAA10154.596425E-47.96600151
CTCTTTT11201.524998E-47.87547871
CTTTTAT11451.9693116E-47.7035251
CTTTTTT21953.2931016E-45.3579571
Produced by FastQC (version 0.11.5)