FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139681
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAT11402.6193447E-910.6320581
CTAAGAA5400.00187491510.5625671
CCCCCCC2101.0753052E-48.11959155-59
CTGCGAT9000.00167845257.92192551
GATTTCG16556.617658E-56.43846135
CGATTTT16755.356368E-45.93747854
CTTCAAT15550.0088523495.50204471
TTTGATT19150.00287076785.20020062
CTTTGAA19950.00464313855.00332161
Produced by FastQC (version 0.11.5)