FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979564
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGTG4050.00238077812.5063574
CTACAAA8004.0295577E-59.9680851
CTTAAAT7050.00141309349.2547021
CTTCCAA8701.0092123E-49.1660571
CTTGAAT8400.00689000787.7673391
CTCAAAA12456.0599804E-46.98748541
GTAATAA10600.00704876856.83916661
CTTCCAG16354.21663E-56.6509331
CTTTGAT12150.00359442556.56334831
CTGGAAA16152.577265E-46.28441051
GAATTTT18553.6683938E-56.24113944
CTTCAAA15450.00103159816.09991651
TTTGATT17801.3985284E-46.0976022
CTTTTCT18000.00103475785.63851261
CTCTTTT17050.003343395.52749061
GATTTTG19250.00247234325.26241545
CTTCGCC19550.00293022655.191471
CTCCTGC18650.0095760795.05328231
Produced by FastQC (version 0.11.5)