FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278122
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAAC13452.8011946E-68.064451
TATAGTC11800.00279126396.7244567
CCACGAT21309.91562E-76.4502981
CCAAGAT16453.384991E-46.15414761
GTCCATT17701.3094032E-46.1280711
CTTTGAT35252.419256E-105.94900941
CCCAAAT15800.0090429235.4919931
CTTCGAA15850.009362515.47466751
CTTCAAC27852.831497E-65.4525531
TCACGAT17700.00551437075.2985213
CTGAAAG45852.2191671E-105.20451641
TCCCGTT23602.1438925E-45.19776732
CTTCAAT37604.0023224E-85.1925621
CTGTAAT23852.4303487E-45.154261
GATATAG22958.1105734E-45.02902465
ATTTGCG20150.0045528375.01186566
Produced by FastQC (version 0.11.5)