FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933309
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3957.894234E-616.1404867
ACACGTC4353.225252E-413.0278248
CCCGGGG3950.002220746612.6226871
CACGTCT4400.005186453511.2698099
GAGCACA5450.002131509110.4005854
AGCACAC5650.002879924610.03027255
GCACACG5750.0033302979.8558336
AGAGCAC6550.0097080118.6539213
CAATTAT9750.00371326837.30543851
CTTTAAA10050.00498811347.0873651
AAATCTT17258.056557E-45.7504684
TTTCAGC22651.5225605E-45.3168197
Produced by FastQC (version 0.11.5)