FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561768
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTGC2500.001425041217.3398579
CCCAACT2650.002048683116.4241391
CCCTCCG3153.4879724E-416.1199871
CTCAAAC3456.962582E-414.7182491
CTCATGG4250.003349993811.9477561
CTTCAAT9702.3102984E-811.2175181
CCCAGAA5200.001183213111.1599921
GTACTGG4800.00849516210.5448188
CCAGAAC6350.0063635469.1103952
CATATTA6350.00636820739.1095843
GTCCTTT6700.0096289258.6614871
CTTTGAT10155.6492456E-58.5761521
CTTTTGT11753.0921536E-47.40833471
CTTTATA9800.0032613047.4020351
CTCCTTT11000.0012493727.2539951
CTTCCAT11150.00144235397.15640741
ATTTAAA11200.00151235917.1244591
TTGATAT13702.466939E-46.86127853
CTTCAAA10850.0087625326.6857091
TCTTCTG12900.0068813776.16574055
Produced by FastQC (version 0.11.5)