FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658297
Sequences flagged as poor quality0
Sequence length100-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA700.00237237268.86186145
CCCCCTT1252.326602E-531.8329351
CACCTCG1400.001679373723.6852231
CCTCGGC2550.002883925815.6043811
TACCGAC3055.589606E-415.15129859
CCTCCTC2800.005371229314.2111331
CCCTGAT3650.002096796612.718641
CTTTAAC7304.3028485E-49.0847431
CTCCTTC7909.4492227E-48.3947611
CTTAAAT9000.00341008637.36873531
CTTCAAA13851.4365007E-57.1825221
CTTTTAT22901.0804797E-96.95042321
CTCCTTT11809.05993E-46.7442661
ACACAGT2900.0083202456.723612125-129
CTTTCTT20801.856862E-55.7391111
Produced by FastQC (version 0.11.5)