FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741041
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATT11653.324749E-89.93417551
CTCACAT8255.795122E-59.6444291
GTTCGGT10450.00628046046.9218391
CTTACAG14354.342957E-46.55283551
CACGCTT12350.0043690746.44263461
CAAACGA12600.0053842616.3148051
CCTGTAT21206.2534345E-66.14149951
GTTCCAC15650.0081115445.54631661
CTTTCGG18900.00197522065.3580161
TAGATAT18450.0091997055.07132243
CCCTGCT18600.0095391825.05554771
Produced by FastQC (version 0.11.5)