FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2546561
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGAT14801.4154157E-67.773021
CTATTTA11400.00206945866.9184184
CTCCTAT15007.9948275E-46.2313711
CTCGAAT13050.0082509356.06057261
CTCGGCT16654.256153E-46.0456821
CCCACTT14800.00467574745.8297651
CCCGAAT22551.8685656E-55.73928071
GATTCGG22100.00257007785.6522703145
AATTTAA19154.295121E-45.61618575
CTTCGAT29602.8783688E-75.5868581
GGTCAAC16850.0032117565.5472141
GTTCTCT22209.0296366E-55.5058891
CCCAGCT18650.00180267675.39735131
CTTCGCG21703.8550718E-45.3014141
CTCAAAA28705.4463762E-65.2610071
CAAAAAT40207.4251147E-95.18684671
CTTCAAT39302.3475877E-85.1226771
CCTCGAA24203.286277E-45.05085661
CTTATTT29951.1799593E-55.04143241
CTCCAAT20050.00445750455.0204791
Produced by FastQC (version 0.11.5)